Molecular Biotechnology- Theory and Application

This course will cover all aspects of Biotechnology including theoretical basis of gene manipulation, products and processes involved in this fastest growing discipline. The usefulness and implications of biotech products will be discussed. The biotech concepts learned in the lectures would be reinforced by hands-on laboratory projects. The lab component of the course will include several techniques such as, genomic and plasmid DNA isolation and purification, from a variety of samples, cloning gene of interest, separation technology, blotting technology, gene library construction and screening, RTPCR technology, DNA fingerprinting technology (RAPD, AFLP, Microsatellite, RFLP) and DNA sequencing and analysis that are routinely used in most molecular biotechnology laboratories. On successful completion of this course students should be able to directly apply these techniques if they decide a career in biotechnology. Students taking this course for the first time are required to take the lecture and the corresponding lab course concurrently. If the lab or lecture component of the course has been successfully completed previously with a passing and transferable grade or better, then the corresponding lab or lecture course that was not successfully completed previously may be retaken separately.

Fall, Spring

024661